5'-Dimethoxytrityl protection group of synthetic oligonucleotide stability in polymerase chain reaction

Stannous Chloride: A Reagent for Removal of Dimethoxytrityl Group from 5'-Dimethoxytrityl Nucleosides

Detritylation of 5'-dimethoxytrityl nucleosides have been quantitatively achieved in minutes at room temperature under aprotic neutral conditions by using stannous chloride. Of additional practical consideration and in contrast to protic acids, no depurination was observed with this reagent.

Detection of Babesia ovis Using Polymerase Chain Reaction.

SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

Diagnosis of group A coxsackieviral infection using polymerase chain reaction.

AIMS To examine the relation between enteroviral infection, especially group A coxsackieviral infection, and acute febrile illness over two summers using tissue culture and polymerase chain reaction (PCR). METHODS Throat swabs were collected from 246 children from June to August 1997 and 1998. RESULTS Enteroviruses were isolated from 33/246 samples and 35 other viruses were isolated. Entero...

Polymerase Chain Reaction for the Diagnosis of Tuberculosis

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...